RES-TEC Application Note 01
Layer-by-Layer Thickness Monitoring

RES-TEC Application Note 02
Protein Surface Interaction

RES-TEC Application Note 03
Glass Transitions in Polymer Films

RES-TEC Application Note 04
Surface Plasmon Imaging

RES-TEC Application Note 05
Surface Plasmon Fluorescence Spectroscopy



Surface Plasmon Resonance (SPR) and Waveguide Spectroscopy are powerful tools for the investigation of processes at surfaces. Using evanescent fields the probing force is restricted to the surface and the measurement remains unperturbed by the medium contacting the surface. This makes plasmons the technique of choice for the investigation of adsorption processes of many other surface reactions.

Some examples:


Label-free interaction analysis

The interaction of proteins with surfaces can be monitored label-free using SPR such that the thicknesses of the protein layers can be determined but also such the kinetics of the process is studied. Learn more on this topic in the RES-TEC Application Note 02 (pdf). You may also combine SPR with fluorescence labelling technique to further enhance sensitivity.

Analyze multilayer build-up

The subsequent adsorption of alternately charged polyelectrolytes (Layer-by-layer technique) has become a powerful and often used technique for the generation of functional polymer films. See how plasmons can help you to understand this process in the RES-TEC Application Note 01 (pdf).

Monitor responsive surfaces

RES-TEC spectrometers can be equipped with a temperature controller either to give you the opportunity to perform your measurements at a well-defined temperature to monitor heat induced changes within your films. See how plasmons can be used to determine the glass transition temperature in polymer films of only a few nanometers thickness. See RES-TEC Application Note 3 (pdf)

Image your surface

Replacing the detector of a RES-TEC SPR with a CCD camera allows you to image your surface microstructure. See how a polymeric grid was visualized in RES-TEC Application Note 4 (pdf)


Surface Plasmon Fluorescence Spectroscopy

Combine plasmons with fluorescence and use the stong field enhancement to detect even minute amounts of an analyte bound to a biosensor surface. See RES-TEC Application Note 5 (pdf)

Want more? Download a list of interesting papers on surface plasmons and the power of evanescent fields: RES-TEC Suggested Reading (pdf).